DNA Sequencing

Long-read sequencing generates fewer but longer DNA reads, making it easier to assemble complete bacterial genomes compared to short-read sequencing. Long-read sequencing is more expensive and produces fewer reads, which can make it appear less valuable superficially, yet it may reveal full genome structure. Modern microbiome science became feasible when DNA sequencing became broadly affordable in the mid-2000s, enabling study of species that cannot be adequately characterized through traditional culturing. Pendulum integrates both long-read and short-read sequencing approaches to achieve a more complete understanding of microbial genomes.