Histopathology

Fluorescence microscopy with auramine-rhodamine staining may provide more sensitive and precise bacillary quantification. Histopathological examination is considered the gold standard for diagnosing and classifying leprosy. Conventional histopathology uses Fite-Faraco staining to demonstrate acid-fast bacilli in skin biopsy specimens. Conventional histopathology has limited sensitivity in paucibacillary leprosy because bacillary burden may fall below detection thresholds. Restricted histopathology sensitivity creates a diagnostic gap for patients who most need early intervention to prevent nerve damage and disability.