Nitric Oxide Testing and Supplementation

The article recommends two capsules of a dietary nitrate supplement once daily for standard cardiovascular and longevity support. Nitric oxide gas cannot be measured directly because it dissipates within milliseconds in the body. Saliva test strips measure salivary nitrite as a validated research proxy for systemic nitric oxide levels. The Berkeley Life protocol includes saliva test strips, a low-oxalate dietary nitrate supplement, and prebiotic nitrate chewing gum. Testing before and 90–120 minutes after nitrate supplementation can show whether nitric oxide levels rise. Nitrate supplementation is described as a foundational daily intervention for adults past their mid-30s.