Senescent HFF-1 Fibroblasts

Senescent HFF-1 cells preserve the stiffness-dependent sequence despite reduced metabolic and migratory activity. The direct experimental dataset is limited to one cell type and one senescent state. Observing the same stiffness-dependent sequence in senescent cells supports the idea that physical constraints dominate over active signaling alone. The study used HFF-1 fibroblasts at passages described as advanced senescent, metabolically limited, and strongly stress-fiber developing.