Trial Measurement and Analysis Methods

Cited microbiome workflow tools include Cutadapt, QIIME 2, SILVA, qiime2R, phyloseq, and Picante. The reference list includes stool analysis and microbiome profiling methods. The cited references include a gastrointestinal symptom scale and a health-enhancing physical activity questionnaire. The supplied citations suggest mixed-effects modeling, community analysis, differential abundance testing, and graphical reporting, but not the exact statistical specifications or trial results. The references suggest the trial likely used established dietary, gastrointestinal symptom, physical activity, and metabolic measurement frameworks. A cited method indicates fecal short-chain fatty acid measurement by liquid chromatography-tandem mass spectrometry.